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HS-LS4-3NGSS*ScienceLife ScienceGrades 9-12

HS-LS4-3: Using Statistics to Show Advantageous Traits Increase

In plain English: HS-LS4-3 is the NGSS high school performance expectation that asks students to apply statistics and probability to support explanations that organisms with an advantageous heritable trait tend to increase in proportion to organisms without it. Students analyze shifts in trait distributions using percents, graphs and simple models. It is usually taught in Biology.

Apply concepts of statistics and probability to support explanations that organisms with an advantageous heritable trait tend to increase in proportion to organisms lacking this trait.

Clarification Statement: Emphasis is on analyzing shifts in numerical distribution of traits and using these shifts as evidence to support explanations.

Assessment Boundary: Assessment is limited to basic statistical and graphical analysis. Assessment does not include allele frequency calculations.

Next Generation Science Standards · Disciplinary Core Idea: Biological Evolution: Unity and Diversity · Official standard

01

Lesson Plan

55-70 min

Overview

Students use numbers to support the explanation that a heritable trait which helps its carriers survive or reproduce tends to become more common. They turn survival probabilities into expected counts, compute the percent of a population that carries a trait in each generation, graph the change, and ask whether a shift in a sample is large enough to trust. The core move is always the same: compare the proportion of individuals with the trait before and after, and connect the shift to a difference in survival or reproduction.

The NGSS Clarification Statement says: "Emphasis is on analyzing shifts in numerical distribution of traits and using these shifts as evidence to support explanations." The Assessment Boundary says: "Assessment is limited to basic statistical and graphical analysis. Assessment does not include allele frequency calculations." Every calculation on this page counts individuals with a visible trait (dark or light, low-plated or fully plated) and uses percents, means, medians, ranges, ratios, simple probability and graphs. Models assume offspring show the same trait as their parents; the genetics of how the trait is inherited, and any allele frequency calculation, are beyond this standard. All data sets are invented and labeled; several are modeled on real studies. Students do the practice of using mathematics and computational thinking to support an explanation.

Learning Objectives

By the end of this lesson, students will be able to:

  • Use survival probabilities to compute the expected number and percent of individuals with a trait after one round of selection
  • Compute and graph the percent of a population with a trait across generations, and describe the shape of the change
  • Use means, medians, ranges and before-and-after graphs to describe a shift in the distribution of a trait
  • Explain why larger samples and repeated trials give more reliable evidence than a single small sample
  • Write an explanation that uses a statistical shift as evidence that a heritable trait is advantageous

Prior Knowledge Required

Students should already be comfortable with:

  • Using simple math to show how natural selection changes traits over time MS-LS4-6
  • The four factors of natural selection HS-LS4-2
  • Summarizing data with dot plots, histograms and box plots HSS.ID.A.1
  • Comparing center and spread of two data sets HSS.ID.A.2
  • Probability as a number from 0 to 1 and expected value as probability times number of trials

Lesson Procedure

55-70 minutes of class time across 5 phases.

  1. Warm-Up5-10 minutes

    Show two statements from an invented moth survey: "Last year, 30 of 100 moths caught were dark. This year, 45 of 150 were dark."

    Warm-Up Prompt

    "More dark moths were caught this year. Did dark moths become more common? What calculation would you do before answering, and what else would you want to know about how the moths were caught?"

    Let students work it out: both years are 30% dark, so the count rose but the proportion did not. Use this to set the rule for the lesson: evidence for selection is a change in proportion, measured with fair samples, not a change in raw counts.

  2. Direct Instruction15-20 minutes

    From survival odds to a shift in proportion. If individuals with a trait survive or reproduce at a higher rate, then after each generation they make up a larger share of the population, as long as offspring resemble their parents in that trait. Probability predicts the expected share; statistics and graphs test whether real data match. Work through the examples in order, and have students write each computation on whiteboards before you reveal it.

    • Probability to expected counts

      A population of 1,000 moths is 10% dark. On soot-darkened bark, a dark moth has a 0.6 chance of surviving to breed and a light moth has a 0.4 chance (invented values).

      Result: Expected survivors: dark 100 × 0.6 = 60, light 900 × 0.4 = 360. Dark share of survivors = 60 ÷ 420 ≈ 14.3%. If offspring resemble their parents, the next generation starts at about 14.3% dark instead of 10%.

    • A model across generations

      Repeat the same survival chances every generation, starting from 10% dark (Diagram 1, solid line).

      Result: The percent dark goes 10, 14.3, 20.0, 27.3, 36.0, 45.8 over five generations and passes 50% in generation 6. The curve is S-shaped: slow while the trait is rare, fastest near 50%, and slow again as the light moths become rare. A smaller advantage (55% against 45%, dashed line) gives the same shape, stretched out.

    • Field data over time

      An invented survey of 150 stickleback fish each time in a lake colonized by fully armored ocean fish (Diagram 2). Low-plated fish have few armor plates.

      Result: Low-plated share: 6 ÷ 150 = 4% in year 0, 12% in year 3, 30% in year 6, 56% in year 9 and 74% in year 12. A steady rise across five large samples is strong evidence that low-plated fish survive or reproduce better in this lake.

    • Chance in small samples

      In a population that is exactly 50% dark, many random samples of 20 moths are taken.

      Result: By probability, about 96% of such samples contain between 6 and 14 dark moths, that is, between 30% and 70% dark. So a single sample of 20 showing 60% dark is not evidence of a change; a shift must be larger than chance variation, or be seen in larger or repeated samples.

    • A predation experiment (rates and ratios)

      Researchers placed 200 light and 200 dark clay model mice on pale sand dunes for three nights and counted models with bite or claw marks: 12 light and 48 dark (invented, modeled on real studies of beach mice).

      Result: Attack rates: 12 ÷ 200 = 6% for light and 48 ÷ 200 = 24% for dark, so dark models were attacked 4 times as often. This supports the explanation that light fur is advantageous on pale sand, which predicts that light mice will increase in proportion there.

    Keep the vocabulary precise: a change from 10% to 14.3% is an increase of 4.3 percentage points. Remind students that every model here counts individuals by trait; how the trait is carried in genes is a separate question, and allele frequency calculations are beyond this standard.

  3. Guided Practice15 minutes

    Pairs analyze invented data on land snails with banded and unbanded shells. Song thrushes break snail shells on favorite stones, so the broken shells around the stones show which snails were eaten.

    Guided practice data: one hedge habitat, one season (invented)
    Shell typeSnails at the start (marked sample)Shells found broken at thrush stones
    Banded20030
    Unbanded20090
    Total400120
    1. Compute the probability that a banded snail and an unbanded snail were eaten.
    2. Of the snails eaten, what percent were unbanded? Compare with the percent of unbanded snails at the start.
    3. Compute the number of each type that survived and the percent of survivors that are banded.
    4. If shell type is heritable and offspring resemble their parents, predict the percent banded in the next generation.
    5. Write a claim with two numbers as evidence.

    Expected results: 15% of banded and 45% of unbanded snails were eaten; 75% of the eaten snails were unbanded compared with 50% at the start; 170 banded and 110 unbanded survived, so about 60.7% of survivors are banded, up from 50%.

  4. Independent Practice15-20 minutes

    Students analyze invented data modeled on grasses that grow on the waste soil around old copper mines, where copper levels poison most plants. Seeds were collected from the mine site at three times and grown on copper-rich soil in a lab.

    Independent practice data (invented)
    Years after mining beganSeedlings testedSeedlings that grew on copper soil
    02006
    2020070
    40200162

    Students compute each percent (3%, 35%, 81%), graph percent against time, describe the shape of the change, and write an explanation of what the shift shows about copper tolerance. Ask: "Why test seeds in a lab instead of just counting the plants growing at the mine?"

  5. Closure5 minutes

    Exit ticket: "A trait was carried by 25% of a sample of 400 animals ten years ago and by 40% of a sample of 400 animals today. Give two statistics you would report, and one more piece of evidence you would need before calling the trait advantageous." Collect and sort answers by whether they mention a survival or reproduction difference.

Differentiation Strategies

For Struggling Students

  • Start every data set with 100 individuals so counts and percents are the same number, then move to other totals
  • Give a calculation frame: "survivors with trait = number with trait × chance of surviving" and "percent = part ÷ whole × 100"
  • Pair each numeric result with a one-line sentence starter: "The share of ___ went from ___% to ___%, which shows ___."

For Advanced Students

  • Ask students to find, with a spreadsheet, how the number of generations needed to go from 1% to 50% depends on the survival ratio, and to explain the pattern
  • Have students run 50 computer trials of a small-population model and report the range of results, then explain why the class average follows the model more closely than any single trial
  • Ask students to design a sampling plan for the stickleback lake that would detect a change of 10 percentage points, and justify the sample size they choose

Assessment Guidance

What to Look For

Check that students compare proportions, not raw counts, and that they compute survivors from probabilities before computing the new percent. Look for students who confuse percentage points with percent change, who treat one small sample as proof, or who describe a trait increasing because individuals needed it. Strong explanations name the statistic, the size of the shift and the sample sizes, and connect the shift to a survival or reproduction advantage.

02

Classroom Activities

3 Activities

1

Dice Selection Simulation

25 minGroups of 3

Each group runs a physical model of selection with 20 beans: 2 black (a trait with a small survival advantage) and 18 white. Dice decide who survives, so every group's result is different, and pooling the class data shows the trend.

Procedure

  • Put 2 black and 18 white beans in a cup. For each bean, roll a die: a black bean survives on 1-4 (probability 4/6), a white bean on 1-3 (probability 3/6).
  • Each survivor reproduces: add one bean of the same color from the reserve for each survivor.
  • If the cup now holds more than 20 beans, draw 20 without looking; the rest are returned to the reserve (the habitat holds 20).
  • Record the percent black after each generation for 6 generations. Enter the results in the class table.

Sample Class Results (invented)

Class mean percent black across 8 groups: generation 0, 10%; generation 2, 16%; generation 4, 26%; generation 6, 37%. The probability model with a survival ratio of 4/6 to 3/6 predicts about 16.5%, 26.0% and 38.4%.

Discussion Questions

  • Did the percent black rise in every generation in your group? Why can a single group go down even when black beans have the advantage?
  • How close is the class mean to the model prediction? Why does pooling 8 groups help?
  • What would the class mean do if black and white beans both survived on 1-3?

Modification for Distance Learning

Students use a random number generator in a spreadsheet in place of dice (a value below 4/6 means a black bean survives) and share their six-generation results in a class form.

2

Spreadsheet Model: How Big Must the Advantage Be?

20 minPairs

Pairs build a spreadsheet that repeats one round of selection many times and use it to answer a question: how many generations does it take for a trait to go from 1% to more than 50% of a population?

Model

  • Columns: generation, number with the trait, number without, percent with the trait. Start with 10 and 990.
  • Each generation: multiply each number by its chance of surviving, then rescale both numbers so the total is 1,000 again (the habitat holds 1,000).
  • Try survival ratios (trait carriers to non-carriers) of 1.1, 1.5 and 2.0, for example 0.55 against 0.50, 0.60 against 0.40, and 0.80 against 0.40.

Expected Results

The trait first passes 50% after 49 generations with a ratio of 1.1, after 12 generations with 1.5, and after 7 generations with 2.0.

Discussion Questions

  • Doubling the survival ratio from 1.1 to about 2.0 cut the time by far more than half. Why?
  • Graph all three runs on one set of axes. What shape do they share?
  • A real population shows a rise from 1% to 50% in about 10 generations. Which ratio fits best, and what data would test it?

Challenge Variation

Add a column that makes the trait a disadvantage after generation 15 (the environment changes). Students predict and then graph what happens.

3

Moth Survey After Clean-Air Laws

25 minGroups of 3-4

Groups analyze invented survey data modeled on the real decline of dark peppered moths in Britain after clean-air laws reduced soot, which let pale lichens return to tree bark. Here the advantageous trait is light color.

Survey Data (invented)

YearMoths caughtDark moths
1960400360
1970360270
1980300156
199032080
200025020

Tasks

  • Compute the percent dark and the percent light for each year, and graph percent light against year.
  • Find the change in percentage points for each decade. In which decade did the light share rise fastest?
  • Write an argument that uses the graph and at least three numbers to support the claim that light moths had an advantage after 1960.

Discussion Questions

  • The light share rose from 10% to 92% over 40 years. Is that shift too large to be explained by sampling chance? Use the sample sizes.
  • The largest single-decade rise came between 1980 and 1990. How does that fit the S-shaped curve from the lesson?
  • What other evidence (for example, a predation experiment) would strengthen the argument?

Modification for Distance Learning

Share the table in a spreadsheet. Groups build the graph together online and record a two-minute voice explanation of their argument.

03

Diagrams & Visual Aids

2 diagrams

Diagram 1: Model of the Percent of Dark Moths Across Generations

0% 20% 40% 60% 80% 100% 0 2 4 6 8 10 12 generation percent of moths that are dark dark survive 60%, light 40% dark survive 55%, light 45%
Both curves start at 10% dark and assume offspring resemble their parents. With survival chances of 60% for dark and 40% for light moths (solid), the dark share passes 50% in generation 6; with 55% against 45% (dashed), it takes 11 generations. Points are computed from the model and drawn to scale. Both curves rise slowly at first, fastest near 50%, and then level off.

Diagram 2: Low-Plated Stickleback in Lake Samples

0% 20% 40% 60% 80% 100% 4% year 0 6 of 150 12% year 3 18 of 150 30% year 6 45 of 150 56% year 9 84 of 150 74% year 12 111 of 150 percent low-plated Low-plated stickleback in a lake sample (invented)
Invented survey data, modeled on real studies of stickleback fish in lakes colonized by armored ocean fish. Each bar is the percent low-plated in a random sample of 150 fish, drawn to scale. The steady rise across five large samples is the kind of shift in a numerical distribution that supports an explanation of selection.

04

Homework Assignment

~30 min

HS-LS4-3 Homework: Statistics as Evidence for Selection

Directions: All data sets are invented for practice. Assume that offspring show the same trait as their parents. Show every calculation, round percents to the nearest tenth, and do not compute allele frequencies.

Part 1: Probability and Proportions (Problems 1-2)

  1. A population of 500 leaf beetles is 20% green and 80% brown. On green leaves, a green beetle has a 0.9 chance of surviving the season and a brown beetle a 0.6 chance. (a) Find the expected number of survivors of each color. (b) What percent of the survivors are green? (c) By how many percentage points did the green share change? (d) Explain what would happen to the green share if the survival chances stayed the same for many generations.
  2. Hind-leg length (mm) was measured in 12 lizards on an island before a ground predator arrived: 38, 40, 41, 42, 42, 43, 44, 45, 45, 46, 47, 49. A year later, 7 of them were still alive, with leg lengths 42, 44, 45, 45, 46, 47, 49. (a) Find the mean, median and range of each group. (b) Draw two dot plots on the same scale. (c) Describe the shift and explain what it suggests about longer legs, noting one limit of a sample this small.

Part 2: Graphs and Samples (Problems 3-4)

  1. After crayfish were introduced to a pond, the percent of snails with thick shells in yearly samples of 200 was 12%, 20%, 33%, 48% and 63% over five generations. (a) Graph the data. (b) Compute the change in percentage points between each pair of generations. (c) Describe the shape of the change and predict the next value, with a reason.
  2. Student A caught 10 moths and found 6 dark (60%). Student B caught 200 moths in the same woods and found 90 dark (45%). A computer drew 1,000 random samples of 10 from a population that is 45% dark, and 262 of those samples had 6 or more dark moths. (a) Which estimate is more reliable, and why? (b) Is Student A's result surprising if the population really is 45% dark? Use the simulation. (c) What should Student A do next?

Part 3: Designing and Critiquing (Problems 5-6)

  1. Design a bean or bead simulation to test the claim: "Even a small survival advantage, such as 10% higher, can make a trait common within about 50 generations." Describe the starting population, how survival is decided, how reproduction and limited space are modeled, how many trials you will run, the data table and the graph you will make.
  2. A student sampled 5 flowers in a meadow last year and found 2 purple (40%). This year she sampled 5 again and found 3 purple (60%). She concludes: "Purple is an advantageous trait because it increased by 20 percentage points." (a) Compute the percent change as well as the percentage-point change. (b) Give two reasons the evidence is weak. (c) Describe better evidence, naming the statistics you would report.

Rubric

CriterionFull Credit (2 pts)Partial Credit (1 pt)
CalculationsExpected counts, percents, means, medians and ranges correct with work shownMethod correct with one arithmetic error
GraphsCorrect graph type, labeled axes with units, accurate scaleGraph present with a missing label or scale error
Reasoning About SamplesConsiders sample size and chance variation before drawing a conclusionMentions sample size without using it
ExplanationUses a shift in proportion or distribution as evidence and links it to survival or reproductionStates the trend but does not connect it to an advantage

05

Quiz: 20 Questions

Interactive, with answers

Instructions

All data sets are invented for practice. Assume offspring show the same trait as their parents, and do not compute allele frequencies. Your score updates as you answer, and Reset quiz clears everything so you or your students can try again.

Multiple choice: pick an option to check it. Short answer: write your answer, then reveal the model answer.

0 of 20 answered · 0 correct

  1. Question 1 of 20 · Multiple Choice

    Which data would best support the claim that a heritable trait is advantageous in a population?

  2. Question 2 of 20 · Multiple Choice

    A population of 400 fish is 25% striped. Striped fish have a 0.8 chance of surviving and plain fish a 0.5 chance. What percent of the survivors are striped, to the nearest tenth?

  3. Question 3 of 20 · Multiple Choice

    In a sample of 180 lizards, 63 have a dark throat patch. What percent have the patch?

  4. Question 4 of 20 · Multiple Choice

    The percent of a population with a trait rose from 20% to 50%. Which description is correct?

  5. Question 5 of 20 · Multiple Choice

    A graph of the percent of a population with an advantageous trait against generation is S-shaped. Why does the curve rise slowly at first?

  6. Question 6 of 20 · Multiple Choice

    Researchers marked and released 120 dark moths, and recaptured 36 of them a few days later. What is the estimated probability that a dark moth survived to be recaptured?

  7. Question 7 of 20 · Multiple Choice

    Four students estimate the percent of dark beetles in a large field. Which sample gives the most reliable estimate?

  8. Question 8 of 20 · Multiple Choice

    A trait gives no survival or reproduction advantage in a large population. What would you expect its percent to do over several generations?

  9. Question 9 of 20 · Multiple Choice

    The forewing lengths (mm) of five butterflies that survived a storm are 30, 33, 34, 36 and 42. What is the mean?

  10. Question 10 of 20 · Multiple Choice

    Which graph best shows how the percent of a population with a trait changed over ten generations?

  11. Question 11 of 20 · Multiple Choice

    In a season, 45% of dark mice and 30% of light mice survived on lava rock. How many times as likely was a dark mouse to survive?

  12. Question 12 of 20 · Multiple Choice

    During a long drought, the percent of finches with large beaks in yearly samples of 300 birds was 30%, 45% and 58% over three generations (invented). Which conclusion is best supported?

  13. Question 13 of 20 · Multiple Choice

    The percent of dark moths in a forest rose over ten years. Which additional evidence would most strengthen the claim that dark color is advantageous there?

  14. Question 14 of 20 · Multiple Choice

    A population is 50% dark. Dark individuals have a 0.8 chance of surviving and light ones a 0.4 chance. If offspring resemble their parents, what percent of the next generation will be dark, to the nearest tenth?

  15. Question 15 of 20 · Short Answer

    An invented lizard population of 250 is 40% long-legged. Long-legged lizards have a 0.7 chance of surviving and short-legged ones a 0.5 chance. Find the number of survivors of each type and the percent of survivors that are long-legged.

  16. Question 16 of 20 · Short Answer

    The percent of a plant population that tolerates salty soil was 5%, 9%, 16% and 27% over four generations after a road began to be salted in winter (invented). Find the percentage-point change for each generation and describe the pattern.

  17. Question 17 of 20 · Short Answer

    In a dice simulation of selection, the eight groups in a class got different results, and one group's trait even decreased. Why does the class average give better evidence than any one group?

  18. Question 18 of 20 · Short Answer

    On dark soil, researchers set out 150 dark and 150 light clay model mice. Predators attacked 15 dark and 45 light models (invented). Compute each attack rate and how many times as often light models were attacked. What does this predict about fur color on dark soil?

  19. Question 19 of 20 · Short Answer

    Before a cold winter, the mean body mass of a sample of 60 sparrows was 25.0 g with a range of 21-29 g. The 24 survivors had a mean of 26.5 g with a range of 23-29 g (invented). Write an explanation that uses these statistics as evidence.

  20. Question 20 of 20 · Short Answer

    Describe how you would gather statistical evidence that a newly common trait (for example, dark coloring in a lizard population) is advantageous rather than a chance result.

0 of 20 answered · 0 correct

06

Frequently Asked Questions

10 Questions

What does HS-LS4-3 mean?

HS-LS4-3 asks students to use statistics and probability to support the explanation that organisms with an advantageous heritable trait tend to become a larger share of their population. Students compute percents, expected counts and simple summaries, graph changes, and use shifts in the distribution of a trait as evidence. The code means High School, Life Science, core idea 4 (Biological Evolution: Unity and Diversity), performance expectation 3.

What is the assessment boundary for HS-LS4-3?

The official Assessment Boundary limits assessment to basic statistical and graphical analysis and excludes allele frequency calculations. So Hardy-Weinberg equations and computing the frequency of an allele are beyond this standard. Percents of individuals with a visible trait, means, medians, ranges, ratios, simple probability and graphs are in scope.

Is HS-LS4-3 a biology or a math standard?

It is a biology standard that uses math. It is usually taught in high school Biology, and it draws on statistics skills from HSS.ID.A.1 and HSS.ID.A.2 (dot plots, histograms, comparing center and spread). The math stays basic: the science is in explaining what the numbers show.

Why do advantageous traits increase in proportion?

Because individuals with the trait survive or reproduce at a higher rate, they leave a larger share of the next generation. If offspring resemble their parents, the trait's percent rises each generation, even though no individual changes. The rise continues as long as the advantage lasts.

What is the difference between a count and a proportion for this standard?

A count is how many individuals have the trait; a proportion is that count divided by the total. Evidence for selection is a change in proportion, because a population that grows can have more individuals with a trait without the trait becoming any more common.

How can students tell if a change is real or just chance?

They look at sample size and repetition. A small sample can swing widely by chance: in samples of 20 from a population that is half dark, the percent dark usually falls anywhere from 30% to 70%. A steady trend across several large random samples, or a matching survival difference in an experiment, is much stronger evidence.

What simulations work well for teaching HS-LS4-3?

Dice or bead simulations, where a roll decides survival and survivors reproduce, let students see both the trend and the chance variation. Spreadsheet models repeat the same calculation for many generations and show the S-shaped curve. Pooling class data shows why averages follow the model better than one trial.

Why is the curve of an increasing trait S-shaped?

When the trait is rare, there are few carriers, so even a strong advantage adds few new ones and the percent rises slowly. Near 50% the change is fastest. As the trait approaches 100%, there are few non-carriers left to replace, so the curve levels off.

Are the peppered moth data real?

The pattern is real: dark peppered moths became common in sooty industrial areas of Britain in the 1800s and declined after clean-air laws in the second half of the 1900s. The specific numbers on this page are invented for practice and labeled as such, so students can compute with clean values.

How does HS-LS4-3 connect to other standards?

It builds on MS-LS4-6, where students first use math to describe natural selection, and on HS-LS4-2, which explains the four factors behind it. It pairs with HS-LS3-3, which applies statistics to the variation of traits, and leads to HS-LS4-4 on adaptation.